LOCUS       GLB1L-pGEc2             7188 bp    DNA     circular     21-SEP-2016
FEATURES             Location/Qualifiers
     misc_feature    complement(6666..6795)
                     /note="3'-ITR"
     misc_feature    complement(6364..6578)
                     /note="BGH pA"
     rep_origin      1137..1804
                     /dnas_title="Col E1 origin"
                     /label=Col E1 origin
                     /vntifkey="33"
     CDS             129..989
                     /dnas_title="AmpR"
                     /label=AmpR
                     /vntifkey="4"
     misc_feature    1998..2127
                     /note="5'ITR"
     misc_feature    2208..2790
                     /note="CMV Promoter"
     misc_feature    2828..2960
                     /note="Artificial Intron"
     misc_feature    5816..6357
                     /note="WPRE"
     misc_recomb     complement(2974..3001)
                     /label=attB1
                     /note="attB1 site"
                     /note="recognized as attB1"
     misc_feature    4995..5017
                     /note="attB2"
     misc_feature    5795..5800
                     /note="BstB1 site"
     misc_feature    5018..5713
                     /note="Super GFP"
     misc_feature    5714..5764
                     /note="AviTag"
     misc_feature    5765..5788
                     /note="His Tag"
     PCR_frame       3011..4994
                     /note="Reading Frame"
                     /set="Set 1"
     misc_feature    3011..4972
                     /note="OPTIMIZEDD GLB1L coding region"
     misc_feature    4973..4994
                     /note="TEV"
     vector          join(5003..7188,1..2991)
                     /source="pGEc2-DEST"
                     /type="Destination Vector"
     clone           2992..5002
                     /source="GLB1L pDONR"
                     /type="Entry Clone"
     source          1..7188
                     /dnas_title="GLB1L-pGEc2"
ORIGIN      
        1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT
       61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA
      121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT
      181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT
      241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT
      301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG
      361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA
      421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA
      481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA
      541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA
      601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA
      661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA
      721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC
      781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC
      841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG
      901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA
      961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT
     1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA
     1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG
     1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA
     1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT
     1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC
     1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA
     1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA
     1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC
     1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA
     1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA
     1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG
     1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC
     1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG
     1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA
     1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA
     1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG
     1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG
     2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG
     2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT
     2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG
     2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT
     2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG
     2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT
     2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT
     2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG
     2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG
     2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC
     2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA
     2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC
     2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC
     2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT
     2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA
     2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC
     3001 TCGCGCCACC ATGGCCCCTA AGAAGCTGTC TTGTCTGAGA AGCCTGCTGC TGCCTCTGAG
     3061 CCTGACACTC CTGCTGCCAC AGGCCGATAC CAGATCCTTT GTGGTGGACC GGGGCCACGA
     3121 CAGATTTCTG CTGGATGGCG CCCCTTTCAG ATACGTGTCC GGCAGCCTGC ACTACTTCAG
     3181 AGTGCCTAGA GTGCTGTGGG CCGACAGACT GCTGAAAATG AGGTGGTCCG GCCTGAACGC
     3241 CATTCAGTTC TACGTGCCCT GGAACTACCA CGAGCCTCAG CCCGGCGTGT ACAACTTCAA
     3301 TGGCTCCAGA GATCTGATCG CCTTCCTGAA TGAAGCCGCC CTGGCCAATC TGCTGGTCAT
     3361 TCTGAGGCCT GGCCCTTACA TTTGCGCCGA GTGGGAAATG GGAGGCCTGC CTTCTTGGCT
     3421 GCTGAGAAAG CCCGAGATCC ACCTGAGAAC CAGCGACCCT GATTTTCTGG CCGCCGTGGA
     3481 TAGCTGGTTC AAGGTGCTGC TCCCCAAGAT CTACCCCTGG CTGTACCACA ACGGCGGCAA
     3541 CATCATCAGC ATCCAGGTGG AAAACGAGTA CGGCAGCTAC AGAGCCTGCG ACTTCAGCTA
     3601 CATGAGACAC CTGGCCGGCC TGTTTAGAGC CCTGCTGGGA GAGAAGATCC TGCTGTTCAC
     3661 AACAGATGGC CCCGAGGGCC TGAAGTGTGG TAGCCTGAGA GGCCTGTACA CCACCGTGGA
     3721 TTTCGGCCCT GCCGACAACA TGACAAAGAT CTTCACCCTG CTGCGGAAGT ACGAGCCACA
     3781 CGGCCCTCTG GTCAATAGCG AGTACTACAC CGGCTGGCTG GACTACTGGG GCCAGAATCA
     3841 CAGCACCAGA TCTGTGTCTG CCGTGACAAA GGGCCTGGAA AACATGCTGA AGCTGGGCGC
     3901 CTCCGTGAAT ATGTACATGT TCCATGGCGG CACCAACTTC GGCTACTGGA ACGGCGCTGA
     3961 CAAGAAGGGC AGATTCCTGC CCATCACCAC CAGCTACGAC TACGATGCCC CTATTAGCGA
     4021 GGCCGGCGAT CCTACACCTA AGCTGTTTGC CCTGCGGGAC GTGATCAGCA AATTCCAGGA
     4081 AGTGCCTCTG GGCCCACTGC CTCCTCCATC TCCAAAAATG ATGCTGGGCC CTGTGACACT
     4141 GCACCTCGTG GGACATCTGC TCGCTTTTCT GGACCTGCTG TGCCCTAGAG GCCCTATCCA
     4201 CAGCATCCTG CCTATGACCT TTGAGGCCGT GAAGCAGGAC CACGGCTTTA TGCTGTACCG
     4261 GACCTACATG ACCCACACCA TCTTCGAGCC TACCCCCTTC TGGGTGCCAA ACAATGGCGT
     4321 GCACGATCGG GCCTATGTGA TGGTGGACGG TGTCTTTCAG GGCGTGGTGG AACGGAATAT
     4381 GCGGGACAAA CTGTTCCTGA CCGGCAAGCT GGGATCCAAG CTGGACATCC TGGTCGAGAA
     4441 CATGGGCAGA CTGAGCTTCG GCAGCAACAG CTCCGATTTC AAGGGCCTCC TGAAGCCTCC
     4501 TATCCTGGGC CAGACAATCC TGACCCAGTG GATGATGTTC CCCCTGAAGA TCGACAACCT
     4561 GGTCAAGTGG TGGTTCCCAC TGCAGCTGCC TAAGTGGCCT TATCCTCAGG CCCCAAGCGG
     4621 CCCTACCTTC TACAGCAAGA CCTTCCCTAT TCTGGGCAGC GTGGGCGACA CATTCCTGTA
     4681 CCTGCCTGGA TGGACAAAAG GCCAGGTCTG GATCAACGGC TTCAACCTGG GCAGATACTG
     4741 GACCAAGCAG GGCCCTCAGC AGACCCTGTA CGTGCCAAGA TTCCTCCTGT TCCCTAGGGG
     4801 AGCCCTGAAC AAGATCACAC TGCTGGAACT GGAAGATGTG CCCCTGCAGC CTCAGGTGCA
     4861 GTTCCTGGAT AAGCCCATCC TGAACTCCAC CTCCACCCTG CACAGAACCC ACATCAATTC
     4921 TCTGTCCGCC GACACACTGA GCGCCAGCGA ACCTATGGAA CTGAGCGGAC ATgaaaactt
     4981 gtactttcaa ggctCCAGCT TTCTTGTACA AAGTGGTATG AGCAAGGGCG AGGAACTGTT
     5041 CACCGGCGTG GTGCCCATCC TGGTGGAACT GGATGGCGAC GTGAACGGCC ACAAGTTCTC
     5101 TGTGCGGGGA GAGGGCGAAG GCGACGCCAC AAATGGCAAG CTGACCCTGA AGTTCATCTG
     5161 CACCACCGGC AAGCTGCCCG TGCCTTGGCC TACCCTCGTG ACCACACTGA CCTACGGCGT
     5221 GCAGTGCTTC AGCAGATACC CCGACCATAT GAAGCGGCAC GACTTCTTCA AGAGCGCCAT
     5281 GCCCGAGGGC TACGTGCAGG AACGGACCAT CAGCTTCAAG GACGACGGCA CCTACAAGAC
     5341 CAGAGCCGAA GTGAAGTTCG AGGGCGACAC CCTCGTGAAC CGGATCGAGC TGAAGGGCAT
     5401 CGATTTCAAA GAGGACGGCA ACATCCTGGG CCACAAGCTG GAGTACAACT TCAACAGCCA
     5461 CAACGTGTAC ATCACCGCCG ACAAGCAGAA GAACGGCATC AAGGCCAACT TCAAGATCCG
     5521 GCACAACGTG GAAGATGGCA GCGTGCAGCT GGCCGACCAC TACCAGCAGA ACACCCCCAT
     5581 CGGAGATGGC CCCGTGCTGC TGCCCGACAA CCACTACCTG AGCACCCAGA GCGTGCTGAG
     5641 CAAGGACCCC AACGAGAAGC GGGACCACAT GGTGCTGCTG GAATTCGTGA CCGCCGCTGG
     5701 CATCACCCAC GGCATGAGCG GCCTGAACGA CATCTTCGAG GCCCAGAAAA TCGAGTGGCA
     5761 CGAGCACCAC CATCATCACC ATCACCACTG ATGATTCGAA AAATAATAAG GATCCAATCA
     5821 ACCTCTGGAT TACAAAATTT GTGAAAGATT GACTGGTATT CTTAACTATG TTGCTCCTTT
     5881 TACGCTATGT GGATACGCTG CTTTAATGCC TTTGTATCAT GCTATTGCTT CCCGTATGGC
     5941 TTTCATTTTC TCCTCCTTGT ATAAATCCTG GTTGCTGTCT CTTTATGAGG AGTTGTGGCC
     6001 CGTTGTCAGG CAACGTGGCG TGGTGTGCAC TGTGTTTGCT GACGCAACCC CCACTGGTTG
     6061 GGGCATTGCC ACCACCTGTC AGCTCCTTTC CGGGACTTTC GCTTTCCCCC TCCCTATTGC
     6121 CACGGCGGAA CTCATCGCCG CCTGCCTTGC CCGCTGCTGG ACAGGGGCTC GGCTGTTGGG
     6181 CACTGACAAT TCCGTGGTGT TGTCGGGGAA GCTGACGTCC TTTCCATGGC TGCTCGCCTG
     6241 TGTTGCCACC TGGATTCTGC GCGGGACGTC CTTCTGCTAC GTCCCTTCGG CCCTCAATCC
     6301 AGCGGACCTT CCTTCCCGCG GCCTGCTGCC GGCTCTGCGG CCTCTTCCGC GTCTTCGAGA
     6361 TCTGCCTCGA CTGTGCCTTC TAGTTGCCAG CCATCTGTTG TTTGCCCCTC CCCCGTGCCT
     6421 TCCTTGACCC TGGAAGGTGC CACTCCCACT GTCCTTTCCT AATAAAATGA GGAAATTGCA
     6481 TCGCATTGTC TGAGTAGGTG TCATTCTATT CTGGGGGGTG GGGTGGGGCA GGACAGCAAG
     6541 GGGGAGGATT GGGAAGACAA TAGCAGGCAT GCTGGGGACT CGAGTTAAGG GCGAATTGCC
     6601 GATTAGGATC TTCCTAGAGC ATGGCTACGT AGATAAGTAG CATGGCGGGT TAATCATTAA
     6661 CTACAAGGAA CCCCTAGTGA TGGAGTTGGC CACTCCCTCT CTGCGCGCTC GCTCGCTCAC
     6721 TGAGGCCGGG CGACCAAAGG TCGCCCGACG CCCGGGCTTT GCCCGGGCGG CCTCAGTGAG
     6781 CGAGCGAGCG CGCAGCCTTA ATTAAGAGCT CATGGCGCGC CTAGGCCTTG ACGGCCTTCC
     6841 GCCAATTCGC CCTATAGTGA GTCGTATTAC GTCGCGCTCA CTGGCCGTCG TTTTACAACG
     6901 TCGTGACTGG GAAAACCCTG GCGTTACCCA ACTTAATCGC CTTGCAGCAC ATCCCCCTTT
     6961 CGCCAGCTGG CGTAATAGCG AAGAGGCCCG CACCGAAACG CCCTTCCCAA CAGTTGCGCA
     7021 GCCTGAATGG CGAATGGGAG CGCCCTGTAG CGGCCACTCA ACCCTATCTC GGTCTATTCT
     7081 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA
     7141 CAAAAATTTA ACGCGAATTT TAACAAAATA TTAACGCTTA CAATTTAG
//

